DNA Isolation from Bacteria

बेटी बचाओ कल हमारा होगा

लड़की परायी धन होती है अपने घर में है तो माँ पापा बोलते है और शादी करके ससुराल जाती है तो ससुराल वाले बोलते है लेकिन लड़की का घर है कहा???? इसके उलट देखिये तो ये दुनिया को जन्म देने वाली भी लड़की ही है वो है धरती माता ! राजा को या गरीब को सबको लड़की ही जन्म देती है फिर भी लड़की परायी है ? भगत सिंह हो या मोदी हो , राम हो या रहीम हो सबको को लड़की ही जन्म दी है फिर उनका अधिकार कंही साबित नहीं होता है ??
अगर बात किया जाये आज़ादी के ६० साल बाद भी तो आज भी लड़की की दशा में उतनी सुधार नहीं ...आई है जितनी होनी चाहिए ! भारत के कुल गावों में सरकारी बिधालयो में देखा जाये तो कंही भी लड़की सौचालय की सुबिधा नहीं है आज भी कितनी लड़कियां पढ़ने में तेज होने के बाद भी
वह स्कूल नहीं जा पाती है क्योंकि उनको वो माहोल नहीं मिल पता है वहा आज भी यतायात की सुबिधा नहीं है ? और अगर कोई लड़कियां पढ़ लेती भी है तो दहेज़ के लोभी माहोल के चलते कुछ उसकी बलि चढ़ जाती है तो कुछ दहेज़ नहीं देने के वजह से ऐसे लड़के के पाले पड़ जाती है या तो वो खानदानी धनि है या खानदानी बुरबक है और बस दहेज का कीड़ा उसके सभी सपने को खा जाता है ? मै पूछना चाहता हूँ आखिर ये लडकिया जाये तो जाये कहा? जबकि टाटा हो या बिरला या फिर बिल गेट सभी ने एक औरत से ही जन्म लिया है ! कितनी लड़कियों को गर्भ में ही मार दिया जाता है !
एक गर्भ में पल रही बेटी माँ से दुआ करती है..
हे माँ मुझे भी आने दो दुनिया में
मै भी रानी लक्ष्मी बन सकती हूँ
मै भी कल्पना चावला बन सकती हूँ
मै अगर ये सब नहीं कर सकी फिर भी
भगत सिंह जैसे पुत्र दे सकती हूँ
मै शायद कोई दूसरा चंद्रशेखर
या स्वामी विवेकानंद दे सकती हूँ
माँ मुझे भी आने दो इस दुनिया में
मत मारो मुझे मै भी सरला ठकराल बन जाऊ या बना दू ...
बेटी बचाओ कल हमारा होगा
बेटी पढ़ाओ देश को आगे बढ़ाओ

Save Water Water is life

Importance of three minute in my Life....at Central Kanpur station plat -9 at 9.40 pm 23 sept.2014

Life is always giving new experience. I am going share about my changed concept of life and my history. it was happened with me when I was travelling Delhi to Bokaro via Dhanbad by Rajdhani express. those days was festival days I was going to India celebrate durga puja with family . 23 sept 2014 my flight (fly Dubai -102) landed at Delhi IGI airport morning 3.50 am after finished my emigration I went to check the hotel near by airport for staying upto evening bcz my train rajdhani express was at evening 5 pm . then I got one Dreamliner hotel near new Delhi station. I had finished my formalities about hotel then I paid 2400 for one day . I got very comfortable room . I was taking rest in between I got  phone one of the my family friend uncle he was asking where r u? I said I m in delhi I m taking rest and waiting for my train which is  at evening. so he said do not worry   I want to meet you, I said ok but why?? he said I m with somebody who is my close friend he want to see you . I said ok I m in hotel. I have given hotel address. while my friend they reached at hotel we are doing lunch in between my uncle friend came with those people his name was BP Singh. he came with wife and son . I had finished my lunch very quick then I have left my friend to home but its not a correct way to leave your friends very urgent if you have invited them and you do not have time to spend with them. I was feeling very guilty. but what could I do? I am sorry my dear all .
I told them and given him my room key 302. they went there . I went there after finished my lunch. suddenly I got messaged from my  mom some body coming to see you they are trying to make new relation by marry his /her daughter. I said they have already seen my self . mr. bp singh he was start to ask question ....this question and answer session took more then 2 hours . then we went down to station for my train Rajdhaani express for Dhanbad- Bokaro . I reached station, as usual everybody know Delhi station is very big, busy and full of  crowd . we can not imagine like this crowd except metro city. evening 5 o clock my train came I got my birthseat ac first class seat no 1.
I would like to tell you some thing about my reservation of train which is very imagine for me . I got waiting list 3 for first class . but I did not get conformation .in same train same class one of the agent given me 134 waiting list ticket with extra charge more then 2 thousand . I got conformation after chart preparation before he said its 100 % sure my waiting list which is no. 3 its gonna be conform if you wish check it do not cancel it I did same really his ticket which is 134 it was confirm but my ticket which is only no.3 waiting list or it was not confirm. what a magic or its a big corruption which is in every department in India?? anyway leave it we will try to fight against corruption .
finally I got my train for BKSC those people came upto station for see off . when train was starting I got one call on my mobile .
Hello: I am Ambrik Singh Kushwaha
How r u?
fine uncle .
Ambrik Singh kushwaha : beta where r u?
me: I m in train going to Bokaro
Ambrik kushwha: which train?
me: Rajdhani express
Ambrik Singh kuswaha: ok I am waiting at Kanpur station it will take  4 hours to reach over by this train .
me: ok uncle I will reach around 9.20 pm .
ambrik Singh kushwaha:  ok take care.

my train got full speed . I have reached Kanpur after 4.30 hours at 9.20 PM. I have seen uncle (Ambrik Singh kushwaha) and aunty both are waiting there . I get down from train and went there I did pranam by touch his leg as well as aunty. then we start talk about me and him self . when I touched the leg really I got little bit energetic power I do not know why. but his face charming was very impressive. he given me information about our religion which is very Interesting . I was knowing earlier but I was not considering but after meeting him I became fully changed. when I left Kanpur station, I do not know I was feeling I lost some thing I should be stay with him some time to know what m I ? who is our ancestor ? whole night I was not sleep well. I was thinking why this man struggling for us. what he is getting ? he is very nice guy . he do not have any expectation from our society . our society is not rich to full fill his requirement. but question is here he does not have any more burden . but he is struggling for us. he want make our society level higher than other. he is  social man . it is my luck I met with him. he was national secretory of  SAMART ASHOK CLUB.
he said to me his next programme in Delhi on 3 rd October 2014. I said sorry uncle I could not come due to my busy schedule . but I had tried to call him but mobile was not in signal range. I had call his home contact no. his daughter sneha had received she said me he is busy for conference now plz call later it was at night 10.pm. I was waiting for morning . I did not get free time to call him and get some information . then 6 oct I thought I will call him and I will tell him I want to meet you and I want to stay with  u for some time . I called his personnel mobile no. his daughter received my phone her voice was interrupting or vibrating , I asked her what happed your voice is vibrating . when I heard her voice  she said daddy is not here...I became fully shocked ....in my mouth there is no word to express sorrow ...sudden I fall down on my bed bcz time was 7.30 am just I had wakeup. I was not understanding what should I do? how its happened? why god is very cruel? who is good man he want took from us. whole day I was very sad. I m yet feeling he is with me . my concept fully changed . I m son of Asoka who is great Indian emperor in the world. I m Buddhist or Hindu its not a matter but I m a son of great emperor. to many people they do not know. what we r ? its symbol of less education or lack of information. 
I will try to continue his journey through my way which is he leaved on the way ...I need his bless or roadmap .foot map

the one he leaved for us like all young boys n girl for our society . I want to know more about our great emperor. I would like to aware my society kushwaha ,saini ,etc... I m missing a lot my ideal icon person Mr. Ambrik Singh Kushwaha. I will write more about his life history later. sir we are missing you but you will be always with us in my /our heart.     

The Celebrating SHAKYA'S: My Origin/My Caste
We are SHAKYA'S / KUSHWAHA “KSHATRIYA SURYAVANSHI” IT’S OUR IDENTITY.
Shakya is a clan of the ancient Vedic period(1500-500 BCE). The name is derived from the Sanskrit word śakya which means capable, able. The genealogy of Shakyas is found in Book IV of the Vishnu Purana, the Bhagavata Purana and the Brahma Purana. The Śākyas formed an independent republican state, known as Sakya Gaṇa-rājya. The Śākya capital was Kapilavastu in pre...sent-day Nepal, which lies near the border of the present-day Uttar Pradesh state of India.[1][page needed] The most famous Shakya was the prince Siddhartha Shakya (5th century BCE) who was the founder of Buddhism and came to be known as Gautama Buddha. Siddhartha was the son of Suddhodana. Suddodhana was the elected leader of Shakya Republic. As Gautama Buddha founded a new religion and abdicated the throne, so the lineage continued with his son Rāhula.
Legend and early history The Kushwaha belong to the Suryavanshi lineage, which claims descent from the Surya and Sun Dynasty of the ancient Kshatriyas. Specifically, they are descent from Kusha younger of the twin sons of Rama. We have rich cultural History &Legacy. World knows about Chandragupta Maura &Samrat Ashoka..

Community. Shakya
Religion: Hinduism
Caste: Kshatriya
Vansh: Suryavansha
Descended from:: Ikshvaku, the grandson of Vivasvan (Surya)
Ruled in: Kapilavastu

 J&K: Saini, Bhaji
Himancal Pradesh: Kushwaha, Saini
Punjab: Saini, Maurya, Kushwaha
Haryana: Saini, Mali, Shakya, Gehlot
Delhi: Saini, Mali, Shakya, Maurya, Kushwaha
Rajasthan: Saini, Mali, Shakya, Maurya, Kushwaha, Kuchhawaha
Gujarat: Saini, Mali, Maurya, Kushwaha, Kacchi
Madhya Pradesh: Kushwaha, Kachhi, Kuchhawaha, Mali, Murar, hardiya, dangi, kavare, Maurya, Mourya
Uttar Pradesh: Koiri, Koiry, Murav, Verma, Bhagat, Kacchi, Maurya, Kushwaha, Mourya, Shakya
Bihar/Jharkhand: Mahto, Bhagat, Maurya, Kushwaha, Singh, Mandal, Dangee, Kapadi, Mehta, Prasad, Shakya ,Dangi
West Bengal: Koiri, Koiry, Kushwaha, Maurya, Mahto
Orissa: Muri, Mali, Phulmali, Bhajmali, Sagberiya
Maharashtra: Kachhiya, Reddy, Phule, Saini, Maurya, Bhujbal, Vankhede, Kushwaha, Mourya
Andhra Pradesh: Phule, Kachhi, Reddy, Nayar
Karnataka: Jeeremali, Phulmali, Nayar, Reddy
Assam: Koiri, Moriya, Mahto, Maurya, Kushwaha

A Day at the Seaside........KSA

The day dawned bright and sunny. It was ideal for a picnic by the sea. We quickly packed some refreshments and swimming costumes, not forgetting our cameras and deck chairs. We are living very near of sea cornice Alkhobar . usually we are a group in three member or you can say 3 idiot but some times no of idiots people were increase occasionally. we did not care about day it is hot or cold whenever we have wished we went there. many time we bath and played bally ball with group in between some guy had discovered new word for pushing the ball ..fingering. everybody were helping to cooking the food but my one of the best friend & most senior man mr. J P suman his lead role in cooking and transportation. At last, we sighted the blue waters, sparkling in the sunlight. We looked out for a clean and shady spot to spend the day as we cruised along. It was a joy to see the graceful coconut palms swaying in the breeze. We saw, too, fishermen's little houses on stilts scattered along the share. Finally, we parked our car at a shady spot under some bushy palms. The white, sandy beach was full of tiny crabs and some desert insect scurrying about. ..continue.... After a while, we swam in the sea which was cool and pleasant. I saw sailing boats in the distance and fishermen busy with their catch. Then we sat on the beach and ate sandwiches, cookies, peanuts and fruit followed by iced drinks. The sun was now fiercely hot, and we felt tired and sleepy as we lay on the sandy beach.... some time we were staying until morning whole night only enjoy with sandy friend continue....

Be the change that you wish to see in the world...............(LIVE HEART)

There are only two ways to live your life. One is as though nothing is a miracle. The other is as though everything is a miracle.We are all in the gutter, but some of us are looking at the stars same like my friend RAJESH KUSHWAHA. Today i am going write here some thing about one of the real live heart man.it is story of Mr.Rajesh Kushwaha who is living with family at Gorakhpur in UP. simple man doing always hrad work with great idea and hard effort. many people doing a lot of work but every work has hidden self benefit,but here zero self benefit did by mr. kushwaha for himself.he is not politician, not businessman, not an actor, He is simple forester police who is protecting our green environment for uncost oxygen.He had arranged the marriage for orphan girl last year at his home town (kusinagar) with help of some gentle people. again we can see ....Humanity is still there, A grand salute to this man & his group team member. f73hXzXcfps/UYjYJ24q4sI/AAAAAAAABhs/yRTChDR28Ok/s320/774078_432130520190317_61159210_o.jpg" /> this year same thing going to repeat by them. girl name is REENA kushwaha who is living in kusinagar ,she's mother & father has died many years ago. here I have attached the marriage card. we know that it is not easy work for everybody. Now a days everywhere & everyday we can hear about abortion by news and electronic media. Today, abortion is a big issue concerning women because for years it had been said that abortion should not be legal.When people talk about abortion, they speak about the power of human being over the nature and that the modern level of science’s development makes it possible to revert usual physical processes.why is it alright to kill him or her before the birth?It is not power over nature, it is power over human beings that do already exist.Abortion has affected nearly everyone. we should try to stop at own level. is it possible? yes, i can say because we need same Rajesh kushwaha or we should become like Mr. Kushwaha then we can save thousand of girl who is going to die before birth. if we are ready to help each other we can change our world through our society. I can not exhibit my happiness through this bolg because i do not have word in my knowledge to say thank you all of you guys who is participating for such kind of work. May god bless them with all the happiness of this world if not now then in his other life, May god solve his all problems.I am very lucky to get chance contribute some small amount for such good work. I proud of my self to get his live friendship.one of the my favorite slogan जिसके नाथ भोले नाथ वो अनाथ कैसे होई !!! so say everybody... her her Mahadev !!! https://www.facebook.com/photo.php?fbid=505231489544262&set=pb.100001722473643.-2207520000.1371408610.&type=3&theater

Failure is the pillar of the success

when I was first time get admission in driving school at kingdom. it had 6 month class for international driving license. I had some experience about driving from India but I was not full of experience so finally I decided will join to school. 31st December 2010 i got admission after 3 month i had one test for driving .i had very good confidence to pass the test but i did not get any positive result, i got fail then i got extra pressure to finish my class within 3 month because this registration is valid for only 3 moth. again i started class with my one of the best trainer from suddan country....normally i was driving very good but when was i going to in front of police to give the test my leg was not moving in correct direction.... i do not know why was i losing my confidence over there. ...this all activity observed by one of the head of police.....he said.. Failure is the pillar of success..do not worry .i had finally decided i would be not able take driving license please arrange one driver for me. i reported to my head office but problem was there how could we get visa for driver because visa process is very hard here..... again i went to school but this time i have decided how ever i have to pass the test for license its does not matter who is stand in front of me ...i got very energetic power from my heart and this time i requested to one of the police man please sit in my car ..i want kick out my police fobia. by luck he was ready to come with me for 1 hour ...i do not know from where i got power, then that day finally i passed my test ... i got my license ...now a days i am driving my car upto 120 km speed at highway....one of the busiest highway in kingdom ..but i can handle .i can drive my car very well .....with out any trouble.....really failure is the pillar of success. Save Water Water is life

My first flight.......

First time i was going out of India by flight ( my snap with Saudi Airlines )... every where were very happiliy moment like at my home, my family , mother , father , sister, brother, grand mother , father and uncle & aunty. everybody was very happy. I was also very happy insidly my heart because i got the job as per my wish research & scientific field. That day was very important for everybody because this is the first time going to happen in my family history... i was going to creat the new history for my future generation so my heart beats were making very enjoyable and glorious moment for me. my self confidence was at high level ...every body blessing me who was with me and who was not with me they were blessing me through phone.

finally we arrived at point to leave the home, my father had arranged one white color car his driver cum owner name is Dinesh singh,he was always parking his car near Neel kamal hotel. we were going through dhanbad bokaro highway NH 21, on the way one of the best temple name BHOOTHNATH TEMPLE it is famous for Bholenath shiva who is one of the strongest god in hindu religoin. i stopped car some time for pray of my safe and happy journey.  see off my sister and my father came at station and my father friend Mr. Lal Babu singh. we reached at time on station,my travel rout from Dhanbad to Delhi( train time 10.30 pm),but my train ticket was not confirmed, unfortunately it was on waiting list. my journey was in night. I have requested with ticket collecter he said he will try than finally i got one seat near GAYA ( Dehri-on-son station) that time morning 4.40 am. i was sleeping for few hours. every thing was going very smoothly. when i have reached KANPUR one story happend with me someone has stolen my new shoes because i had upper berth so i could not watched my shoes properly, i have asked to many people no one said i have seen, finally i took out my sleeper from my carry beg. night 9.30 i have reached on delhi station then i went by Metro to Noida city centre where my uncle is living i hold one night because after one day my flight was from delhi to dammam saudi arab. my uncle name is Jitender kumar. next day i went to collect my visa and Passport from travel agent who did my visa stamping from royal embassy saudi arabia at delhi, when i was submited for stamping he said to me some another amount but when i went to collect my visa he was saying another amount i mean fee of visa stamping, i finally said him i do not have much money to pay you because begining you told me another amount so i do not have extra money, what should i do? tell me. finally i have given same amount plus some extra charges then he accpeted his mistake he was saying some staff did mistake to explain you about the charges of visa stamping fees. after getting the visa i went my uncle home at night. next day morning was very important for me i was going to write new history in my family. next day i wokeup early morning after bath i did my pray for safe and happy journey. me and my uncle together we went by AC Green bus from noida to Airport that time fare was 56 rupees for one person. my uncle paid money for me and him self. we reached within hours 1.30 minute at Airport Delhi.when i was reached at airport little bit i got fear regarding new country and new people. but i had believe that every where god/allah is present so i could not get nervous.my flight time was 6.00 PM so i entered airport around 3.30 pm. i went to my airlines saudi arabia counter i showed my electronic ticket then she gave me my boarding pass and she said to me go there first check your visa with our manager. i went there he checked and he said every thing is ok now you can go for emigration department for exit stamp, after geting the stamp i went to gate no. 21 there is my flight counter.i was entering inside the flight one of girl ( air hostes) she was standing infront of gate and she was saying WEL COME WELCOME sir/mam. i got my seat near the window. while flight was on runway it was very tough for me because suddenly i remembered my family member...situtation was very emotional i controled my self very well. finally i had reached dammam airport in saudi arabia at 10.00 PM local time( Indian time 12.30 AM) one of the representative Mr. Redha was waiting for me. he is very nice man. he asked me how was your journey i said him very good. i reached my unversity compound at 12.30 AM local time then i informed my family that i have reached safely. i talked my mother and brother. next day i met my one of the indian friend Mr. bhasa who helped me to finished the official formalities. finally i had finished my all formalities than started my work .....thank u everybody to read my story.....and thanks a lot everybody who helped me in india and saudi arabia......